Multiplex in situ hybridization chain reaction reveals the spatial organization of six pituitary hormone mRNAs in the Japanese eel

Key Points

• The spatial organization of six hormone mRNAs was revealed using in situ hybridization techniques, enhancing understanding of hormonal regulation.
• Key evidence shows distinct localization patterns for these mRNAs, with implications for hormonal function across tissues.
• Analysis employed multiplex in situ hybridization to examine mRNA distributions in various regions of the pituitary gland of the Japanese eel.
• Findings call for deeper exploration into hormonal signaling, and the unique aspects of the Japanese eel's physiology.