Chicken intestinal epithelial cells (cIECs) play a crucial role in nutrient absorption and maintaining barrier integrity but are highly susceptible to oxidative stress. This study investigated the efficacy of arginine (Arg), tryptophan (Trp), and threonine (Thr) in protecting cIECs from hydrogen peroxide (H₂O₂) induced oxidative stress. The experimental design consisted of 8 treatment groups: a positive control (PC, cells incubated with fresh medium), a negative control (NC, cells exposed to 1 mM H2O2), and 6 additional groups pretreated with either 250 or 500 mM Arg, Trp, or Thr for 24 h (6 replicates per treatment) prior to 6 h incubation with 1 mM H2O2. The results demonstrated that all amino acid treatments significantly improved (p p p p p p p zonula occludens-1, claudin, occludin, and junctional adhesion molecule 2, was greater (p p claudin and occludin compared with NC. In conclusion, Arg, Trp, and Thr attenuate oxidative stress in cIECs by enhancing cell viability, antioxidant capacity, and barrier function, primarily through upregulation of tight junction-related gene expression.
Park et al. (Thu,) studied this question.