This research develops PC-mGFP, a photoconvertible green fluorescent protein for cellular imaging, suggesting new imaging techniques.
A photoconvertible fluorescent protein based on PAGFP, named PC-mGFP, is developed using molecular evolution in a bacterial system. PC-mGFP undergoes photoconversion from the green fluorescent state with a large Stokes shift to the green fluorescent state when irradiated with 405-nm light. The properties of PC-mGFP are characterized in vitro and in the cytosol of mammalian cells. PC-mGFP can be applied in the field of cellular imaging.
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Kuzmicheva et al. (2025) studied this question.
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