The present study focuses on the formulation and evaluation of a herbal hair serum and its preliminary phytochemical, physicochemical, spectral, antimicrobial, and hair regrowth activities. Preliminary phytochemical screening revealed the presence of alkaloids, carbohydrates, tannins, flavonoids, glycosides, saponins, and phytosterols, while proteins, terpenoids, and phenols were absent. Evaluation parameters indicated that the serum was dark brown in color, aromatic, smooth in texture, and had a pH of 8.2 with no irritation potential. The specific gravity (1.096), viscosity (0.91 cps), saponification value (291.72), and spreadability (9 cm) suggested suitable formulation characteristics. UV–Visible spectrophotometric analysis showed maximum absorbance (0.870) at 296 nm, identified as λmax, with negligible interference in the visible region. IR spectral analysis confirmed the presence of functional groups corresponding to bioactive compounds. The formulation exhibited significant antifungal activity against Trichophyton spp. (10 mm) and Candida albicans (12 mm), and antibacterial activity against Staphylococcus aureus (10 mm) and Escherichia coli (22 mm). Hair regrowth studies demonstrated comparative effectiveness with standard treatment, indicating the potential of the herbal hair serum as a natural therapeutic alternative.
Haripriya et al. (Sun,) studied this question.