Hydrogen peroxide (H2O2) is an indispensable mediator in cellular signaling pathways, playing a multifaceted role in biological systems. The development of advanced catalysts derived from flexible sensors for the real-time monitoring of dynamic H2O2 in vivo remains an important research issue. Recently, dual single-atom catalyst-based nanozymes exhibit synergistically enhanced catalytic performance, displaying excellent potential for H2O2 detection. Nevertheless, rationally regulating the geometric structures and electron transfer between the two metals still pose significant challenges. Hence, in this study, an advanced Fe/Ce dual single-atom catalyst (Fe/Ce-C)-based nanozyme was constructed, which proposes a Ce single-atom doping strategy to alter the electronic structure of Fe single-atom sites, endowing it with a high adsorption energy for hydroxyl groups to reduce the energy barrier of H2O2 in the reaction. This Fe/Ce-C achieves a linear sensing behavior in the range of 0.5-10,000 μM with a response speed of 1.4 s, a detection limit of 0.223 μM, and a sensitivity of 213 μA/mM·cm2. Furthermore, the nanozyme-derived flexible electrochemical sensor was assembled for real-time monitoring of H2O2 concentration changes in the brains of living rats, exhibiting long-term detection capability and excellent in vivo biocompatibility. Such an Fe/Ce dual single-atom nanozyme-based electrochemical sensor can serve as a promising tool for monitoring diseases like Parkinson's.
Sun et al. (2026) studied this question.