What does this research mean for the field?

Glycerol stabilizes the tetrameric state of pig heart L-lactate dehydrogenase (LDH) relative to the dimer state by approximately 3 kJ/mol at ambient pressure, but this stabilizing effect declines and shifts toward destabilization at pressures above 40 MPa. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This study aims to understand how glycerol affects the dissociation dynamics of tetrameric LDH under high pressure.

March 6, 2026Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsOpen Access

Effect of glycerol on the pressure dissociation of tetrameric LDH using high-pressure native PAGE velocity method

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This study aims to understand how glycerol affects the dissociation dynamics of tetrameric LDH under high pressure.
Utilized high-pressure native PAGE velocity method
Investigated dissociation process of pig heart L-lactate dehydrogenase
Analyzed tetramer-to-dimer and dimer-to-monomer dissociation steps
Measured effects of 10% glycerol on dissociation pressure
Glycerol affected the dimer-to-monomer dissociation step, increasing pressure requirement
Tetramer-to-dimer dissociation pressure remained unchanged
Glycerol stabilized the tetramer by approximately 3 kJ/mol at ambient pressure
Stabilizing effect of glycerol declined with pressure, becoming destabilizing above 40 MPa

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Implication

High-pressure investigation shows glycerol influences LDH dissociation steps, suggesting stabilization effects.

Cite This Study

Ishiguro et al. (2026) studied this question.

synapsesocial.com/papers/69aa6f0d531e4c4a9ff591f0 https://doi.org/10.1016/j.bbapap.2026.141136

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Effect of glycerol on the pressure dissociation of tetrameric LDH using high-pressure native PAGE velocity method

Key Points

Discussion

Implication

Cite This Study

Effect of glycerol on the pressure dissociation of tetrameric LDH using high-pressure native PAGE velocity method

Key Points

Discussion

Implication

Cite This Study

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