What does this research mean for the field?

Lon protease influences the secretome composition in Klebsiella pneumoniae, affecting its extracellular protein profile. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The research investigates how Lon protease affects the protein profiles in the extracellular environment of Klebsiella pneumoniae.

March 6, 2026Microbiology Resource Announcements0 citationsOpen Access

Lon protease-mediated secretome profiling in Klebsiella pneumoniae

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CRChelsea ReitzelUniversity of Guelph JGJennifer Geddes-McAlisterUniversity of Guelph

Key Points

The research investigates how Lon protease affects the protein profiles in the extracellular environment of Klebsiella pneumoniae.
Applied bottom-up proteomics to analyze proteins
Compared wild-type and Δlon variants of Klebsiella pneumoniae
Examined the secretome of both strains
Identified distinct protein profiles between wild-type and Δlon strains
Highlighted the role of Lon in biofilm formation and antibiotic resistance
Revealed insights into iron response and protein transport mechanisms

Abstract

ABSTRACT Lon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In Klebsiella pneumoniae , Lon has roles in iron response, protein transport, and membrane assembly. Here, we apply bottom-up proteomics to identify proteins in the extracellular environment of WT and Δ lon K. pneumoniae .

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Cite This Study

Reitzel et al. (2026) studied this question.

synapsesocial.com/papers/69aa7066531e4c4a9ff5a183 https://doi.org/10.1128/mra.00311-25

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March 6, 2026Microbiology Resource Announcements0 citationsOpen Access

Lon protease-mediated secretome profiling in Klebsiella pneumoniae

View Full Paper

CRChelsea ReitzelUniversity of Guelph JGJennifer Geddes-McAlisterUniversity of Guelph

Key Points

The research investigates how Lon protease affects the protein profiles in the extracellular environment of Klebsiella pneumoniae.
Applied bottom-up proteomics to analyze proteins
Compared wild-type and Δlon variants of Klebsiella pneumoniae
Examined the secretome of both strains
Identified distinct protein profiles between wild-type and Δlon strains
Highlighted the role of Lon in biofilm formation and antibiotic resistance
Revealed insights into iron response and protein transport mechanisms

Abstract

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View Full Paper

Cite This Study

Reitzel et al. (2026) studied this question.

synapsesocial.com/papers/69aa7066531e4c4a9ff5a183 https://doi.org/10.1128/mra.00311-25

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