Optimization of the extraction process for italicZiyin Zhixuan Formula/italic （滋阴止眩方） based on analytic hierarchy process combined with Box-Benhnken response surface methodology

Key Points

• The aim is to optimize the extraction process for Ziyin Zhixuan Formula based on traditional methods.
• Utilized HPLC and UV-visible spectrophotometry for analysis.
• Determined weights of indicators: yield, gastrodin, and total sugar content using analytic hierarchy process.
• Examined extraction time, water volume ratio, and number of extractions utilizing Box-Behnken design and response surface methodology.
• Weights for yield, gastrodin, and total sugar content identified as 0.539, 0.2972, and 0.1638, respectively.
• Optimal extraction conditions included using 18 times the water volume, extracting for 1.5 hours, and performing the extraction three times.
• The relative standard deviation (RSD) between results and predicted model values was 0.23%, indicating good predictive quality of the model.