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March 6, 2026Scientific ReportsOpen Access

Development of a scalable production bioprocess for HIV-1 virus-like particles coupling continuous VLP harvesting with end-to-end downstream processing

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Authors

ELElianet LorenzoJLJesús Lavado-GarcíaPPPol Pérez-Rubio

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Overview

Demonstrates enhanced Gag VLP production in a scalable process, suggesting improvements for vaccine manufacture.

Key Points

  • The aim is to develop a scalable bioprocess for producing HIV-1 Gag virus-like particles (VLPs) that addresses production and purification challenges.
  • Combined perfusion-based upstream production with a three-step downstream processing.
  • Utilized transient gene expression for VLP production.
  • Implemented secondary clarification and anion exchange chromatography for DSP.
  • Achieved a 2.4-fold increase in VLP volumetric productivity.
  • Obtained approximately 60% recovery and purity after downstream processing.
  • Post-purification lyophilization maintained VLP integrity and stability.

Cite This Study

Lorenzo et al. (2026) studied this question.

synapsesocial.com/papers/69aa70e7531e4c4a9ff5b268https://doi.org/10.1038/s41598-026-41596-y
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