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March 8, 2026

A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.

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Authors

ASAnna StoibSSSahar ShojaeiCSChristine Siligan

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Overview

This assay quantifies urea and ammonia transport in Saccharomyces cerevisiae deletion strains, indicating membrane channels' function.

Key Points

  • To characterize urea and ammonia transport through membrane channels using a yeast complementation assay.
  • Developed a liquid culture method for yeast complementation assays.
  • Used Saccharomyces cerevisiae deletion strains to monitor growth with urea or ammonia as the nitrogen source.
  • Assessed permeability and pH dependency of bacterial urea channel HpUreI and human hAQP8.
  • Demonstrated functional complementation evidenced by cell growth in selective medium for both urea and ammonia transport.
  • Generated solute-specific permeability profiles and pH-dependency comparisons.
  • Showed adaptability for high-throughput formats and diverse solutes.

Cite This Study

Stoib et al. (2026) studied this question.

synapsesocial.com/papers/69acc5b032b0ef16a40504dfhttps://doi.org/10.1002/cpz1.70336
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