The study demonstrates that ultrasound combined with microbubbles promotes wound healing in diabetic rats by regulating macrophage polarization, suggesting new therapeutic approaches.
Key Points
This research investigates the effect of ultrasound with microbubbles on diabetic wound healing, focusing on macrophage polarization.
Established diabetic rat wound models divided into three groups: Model, Ultrasound (US), and Ultrasound Combined with Microbubbles (USMB).
Evaluated wound healing rate, histological examinations, and immunohistochemical staining.
Used immunofluorescence staining and qRT-PCR to detect macrophage polarization and inflammatory factors.
Performed transcriptomic sequencing on wound tissues to analyze gene expression.
Validated pathway-related molecules using qRT-PCR and Western blotting.
Observed increased collagen deposition and stimulated cell proliferation in the USMB group.
The USMB group displayed decreased M1-type macrophage marker CD86 and pro-inflammatory cytokines, while M2-type marker CD206 and anti-inflammatory cytokines increased.
Transcriptomic analysis revealed 1725 differentially expressed genes with significant enrichment in the IL-17 signaling pathway.