The Diagnostic Value of Metagenomic Next- and Third-Generation Sequencing on Cervical Lymph Node Tuberculosis.

Key Points

• This research aims to evaluate the diagnostic accuracy of various sequencing methods for cervical lymph node tuberculosis.
• Recruited 101 patients with suspected cervical lymph node tuberculosis.
• Collected samples using ultrasound guidance.
• Compared diagnostic results of culture, X-pert, metagenomic next-generation sequencing (mNGS), and third-generation sequencing (mTGS).
• Analyzed the diagnostic performance relative to clinical diagnosis.
• 76 cases diagnosed as lymph node tuberculosis, 20 as non-lymph node tuberculosis.
• Metagenomic sequencing methods demonstrated significant diagnostic capabilities.
• Comparison revealed variances in accuracy and sensitivity among the four diagnostic approaches.