Authors clarify protein identification and binding roles in biofilm formation, indicating important structural insights.
The authors note that language regarding the proteins identified in the study should be clarified.The second sentence of the third paragraph of the Introduction should read "Here, we identified GluP, a glutamate-specific TAXI/TRAP protein, as a highly abundant component of the biofilm matrix proteome."In addition, under the heading "GluP Binds Glutamate, and This Binding Is Crucial for Its Function in Biofilm Formation" in the Results section, the second sentence of the third paragraph should read, "To gain a better understanding of GluP function, we chose a structural approach comparing GluP and VCA0144.The structure of GluP bound to ligand has been reported previously, establishing it as a solute receptor belonging to the TAXI family (IPR011852) component of a TRAP transporter (42).The current study is consistent with the previous report, extends the mutational analysis, and enables a comparison with the structure of VCA0144.The structures are similar to that of a solute receptor from a thermophilic organism that was determined bound to either L-glutamate or L-glutamine (41)."
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