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March 12, 2026Journal of Advanced Research0 citationsOpen Access

MaEIL9-MaZIP5-MaSCL8 Module Regulates Banana Fruit Ripening

The MaEIL9-MaZIP5–MaSCL8 module integrates MaBEL1 and synergistically modulates banana fruit ripening

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Authors

FLFan LiuXSXueli SunOSOu Sheng

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Overview

Uncovers the metabolic regulation of banana ripening through the MaEIL9-MaZIP5-MaSCL8 module, suggesting potential for improved fruit quality.

Key Points

  • To elucidate the regulatory mechanisms of MaSCL8 in controlling banana fruit ripening.
  • Yeast one-hybrid and dual luciferase reporter assays to identify downstream targets
  • Electrophoretic mobility shift assays for gene regulation analysis
  • Luciferase complementation imaging to verify protein interactions
  • Stable and transient overexpression assays in tomato and banana
  • MaSCL8 acts as a nuclear-localized transcriptional activator for ripening-related genes.
  • Overexpression of MaSCL8 enhances ripening processes in both tomato and banana.
  • MaEIL9 and MaZIP5 positively regulate MaSCL8's expression through direct promoter interaction.
  • MaSCL8 interacts with MaBEL1, amplifying its regulatory effects on target genes.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/69b2586696eeacc4fcec80fchttps://doi.org/10.1016/j.jare.2026.03.001
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