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March 12, 2026Analytical Methods2 citations

Isothermal Amplification and CRISPR/Cas One-Pot Detection Systems: Strategies and Prospects

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BMBaoying MaXWXiaodong WangXCXiangjing Cai

Key Points

  • To explore the effectiveness of isothermal amplification and CRISPR/Cas systems for detecting nucleic acids of pathogens.
  • Review of traditional and modern molecular diagnostic techniques
  • Evaluation of isothermal amplification and CRISPR/Cas systems
  • Comparison of sensitivity and specificity across methods
  • Isothermal amplification methods increase sensitivity for pathogen detection
  • CRISPR/Cas systems provide high specificity in detecting nucleic acids
  • Combined methods show potential for precise diagnostic applications

Abstract

Molecular diagnostic techniques, known for their high sensitivity and specificity, are now the gold standard for detecting pathogen nucleic acids and are crucial in precision medicine. Traditional methodologies, including polymerase...

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Cite This Study

Ma et al. (2026) studied this question.

synapsesocial.com/papers/69b25b1996eeacc4fcec9777https://doi.org/10.1039/d5ay01976a
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