Addressing Analytical Challenges to Improve Laboratory Consistency in Unbound Flucloxacillin Measurement

Key Points

• This research aims to address analytical challenges in measuring unbound flucloxacillin (FLU) using advanced methods.
• Developed an LC-HRMS method for quantifying unbound FLU
• Conducted cross-validation to assess consistency across laboratories
• Investigated the effects of UF parameters, such as device type and temperature
• Recommended plasma matrix storage for improved sample stability.
• Achieved comparable FLU measurement results across laboratories with harmonized UF parameters
• Found that unbound FLU stability is limited in ultrafiltrate
• Recommended use of a plasma matrix for better sample storage and stability.