Biological Activities and LC–QTOF–MS-Based Phytochemical Characterization of Onosma alboroseum Fisch. et Mey. subsp. alboresum var. alboroseum Extracts and Extract-Loaded Nanoparticles
Evaluates antioxidant and antimicrobial activities of Onosma alboroseum, suggesting nanoparticle formulations enhance certain effects.
Key Points
The research aims to evaluate the biological activities and phytochemical properties of Onosma alboroseum extracts, focusing on nanoparticle formulation effects.
Assessed antioxidant activities using DPPH, FRAP, and CUPRAC assays.
Determined total phenolic content via Folin–Ciocalteu method.
Evaluated antimicrobial activity using agar well diffusion and microdilution assays.
Conducted enzyme inhibitory assays through anticholinesterase and anti-urease tests.
Performed LC–QTOF–MS metabolite profiling of the most active extract, followed by nanoparticle formulation.
The methanol extract had the highest total phenolic content and demonstrated strong antioxidant, antimicrobial, and enzyme inhibition activities.
LC-QTOF-MS profiling revealed a phenolic-rich composition with rosmarinic acid being the main compound.
Nanoparticles increased cholinesterase and urease inhibition (28.03% and 12.11%, respectively) and improved antibacterial efficacy with MIC ranging from 3.13 to 12.5 µg/mL.