Structure-guided engineering of membrane-binding regions for surfactant-free solubilization of direct electron transfer-type alcohol dehydrogenase

Key Points

• The aim is to enhance the bioelectrocatalytic efficiency of membrane-bound alcohol dehydrogenase for ethanol oxidation.
• Predicted membrane-binding regions of alcohol dehydrogenase (ADH)
• Constructed modified ADH with improved binding regions
• Evaluated effects on bioelectrocatalysis
• Enhancements in the solubilization of modified ADH were observed
• Increased efficiency in ethanol oxidation
• Demonstrated potential for improved biocatalytic applications