What does this research mean for the field?

The self-assembling peptide P11-4 (Curodont™ Protect) enables near-complete microhardness recovery of enamel after bleaching, outperforming other remineralization agents. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This study evaluates the effectiveness of various remineralization agents on enamel microhardness and color stability post-bleaching.

March 17, 2026Open Access

Comparison of the effect of self-assembling peptide P11-4 on post-bleaching shade stability and microhardness with different bioactive remineralization agents

Key Points

This study evaluates the effectiveness of various remineralization agents on enamel microhardness and color stability post-bleaching.
Thirty caries-free premolar specimens were sectioned for enamel testing.
Microhardness and color measurements were taken before and after bleaching and after treatments.
Six remineralization protocols were compared over 28 days following a standardized bleaching process.
Bleaching reduced enamel microhardness significantly across all groups.
Curodont™ Protect achieved microhardness recovery comparable to baseline, outperforming other agents.
No significant differences in color change stabilization were noted among the groups.

Abstract

This in vitro study aimed to evaluate and compare the efficacy of six remineralization protocols—including a self-assembling peptide (SAP) P11-4 (Curodont™ Protect), a surface pre-reacted glass-ionomer agent (PRG Pro-Care Gel), nano-hydroxyapatite (Apa Care Repair), casein phosphopeptide-amorphous calcium phosphate (Tooth Mousse), fluoride (ReminPro), and a control—on enamel microhardness recovery and color stability following in-office bleaching with 35% hydrogen peroxide. Thirty caries-free premolars were sectioned to obtain enamel specimens. Baseline (T0) color (CIEDE2000) and microhardness (Vickers, 300 g/15s) were measured. All samples underwent a standardized bleaching protocol (three 8-minute cycles, total 24 min). Post-bleaching measurements (T1) were taken. Specimens were randomly assigned to six groups (n = 5): Control (artificial saliva), ReminPro, Tooth Mousse, Apa Care Repair, PRG Pro-Care Gel, and Curodont™ Protect. Remineralization agents were applied four times over 28 days. Final measurements (T2) were taken after 28 days in artificial saliva. Data were analyzed using Two-Way Repeated Measures ANOVA with Bonferroni post-hoc tests (α = 0.05). Bleaching significantly reduced enamel microhardness in all groups (p PRG Pro-Care Gel > Apa Care Repair ≈ Tooth Mousse > ReminPro > Control. For ΔE, a significant time effect was observed (p < 0.001), but no significant intergroup differences were found (p = 0.726). A significant interaction was found for ΔL (p = 0.020), with Curodont™ Protect showing final lightness statistically indistinguishable from baseline. The choice of remineralizing agent influences enamel recovery after bleaching, particularly regarding mechanical properties. While all agents provided comparable ΔE stabilization, SAP P11-4 was uniquely effective, enabling near-complete microhardness recovery and distinct return of lightness to baseline. S-PRG and nano-hydroxyapatite also demonstrated strong efficacy. Within the limitations of this in vitro study, biomimetic agents such as SAP P11-4 may offer advantages for structural recovery and shade perception following in-office bleaching. Further clinical studies are warranted to confirm these findings.

اسأل الذكاء الاصطناعي

Bookmark

View Full Paper

Cite This Study

Aşık et al. (Sat,) studied this question.

synapsesocial.com/papers/69b8f0f0deb47d591b8c58ea https://doi.org/https://doi.org/10.1186/s12903-026-08091-2

اسأل الذكاء الاصطناعي

Bookmark

View Full Paper