Decoding the enzymatic basis for structural diversity of lycibarbarspermidines, a major class of bioactive compounds in wolfberry

<x>Graphical abstract</x> <x>Highlights</x> Filling the knowledge gap of plant phenolamide glycosyltransferases Elucidating the enzymatic basis of lycibarbarspermidine structural diversity in wolfberry Unraveling the regioselective catalytic mechanism of the lycibarbarspermidine glycosyltransferases <x>In Brief</x> This commentary evaluates the study that first identified five novel phenolamide glycosyltransferases (LbUGT1–5) from wolfberry, elucidating the enzymatic basis underlying the structural diversity of its major bioactive lycibarbarspermidines and the regioselective catalytic mechanism of these enzymes, thus providing valuable biochemical evidence for the geo-herbalism of wolfberry.