Why the study?
Does cell culture and passaging alter gene expression patterns and proliferation rates in rheumatoid arthritis synovial fibroblasts?
Population
Rheumatoid arthritis synovial fibroblasts (RASF)
Comparison
Cell culture and passaging vs Early culture passages
Design
Preclinical
Authors
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Passaging alters RASF gene expression and may bias results; leaves open whether early-passage standardization improves reproducibility in RA models.
Does cell culture and passaging alter gene expression patterns and proliferation rates in rheumatoid arthritis synovial fibroblasts?
Cell culture and passaging alters gene expression in rheumatoid arthritis synovial fibroblasts, suggesting experiments should be limited to early passages to avoid false positive results.
Neumann et al. (2010) studied this question.
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