What question did this study set out to answer?

This study aims to investigate the utility of talaporfin sodium fluorescence in evaluating invasive tumor cell infiltration in glioblastoma.

March 21, 2026Surgical Neurology International0 citations

Quantitative spectroscopic analysis of talaporfin sodium fluorescence: Utility in fluorescence-guided surgery and detection of invasive tumor cells in glioblastoma

SOSho OhtsukaEhime University AIAkihiro InoueEhime University HWH WATANABEEhime University

Key Points

This study aims to investigate the utility of talaporfin sodium fluorescence in evaluating invasive tumor cell infiltration in glioblastoma.
Included 12 patients with recurrent glioblastoma for surgical resection and PDT with talaporfin sodium.
Classified tumors into high-invasive and low-invasive subtypes using magnetic resonance imaging.
Analyzed TS-fluorescence intensities in peritumoral areas using fluorescence spectroscopy.
Correlated fluorescence intensities with tumor cell activity assessed by Ki-67 staining index.
Identified five high-invasive and seven low-invasive tumors among the subjects.
Patients with low-invasive glioblastoma had significantly longer progression-free and overall survival.
Median Ki-67 staining indices varied with fluorescence signal intensity, indicating correlation with tumor density.
No tumor cells were detected when TS signal intensity was below 1000 arbitrary units.

Abstract

Background: Talaporfin sodium (TS) has been widely used as photodynamic therapy (PDT) for glioblastoma (GB), but its sensitivity and selectivity in fluorescence-guided surgery are not fully understood. This study aimed to elucidate whether quantitative analysis of spectroscopic TS fluorescence intensity could evaluate the degrees of glioma cell infiltration in the peritumoral area. Methods: The study included 12 patients with recurrent GB who underwent surgical resection and PDT with TS. Primary tumors were classified into high-invasive (HI) and low-invasive (LI) subtypes based on magnetic resonance imaging. TS-fluorescence intensities were analyzed using fluorescence spectroscopy. A relationship between TS-fluorescence intensities that were measured in four subareas divided according to the signal intensity and degrees of tumor activity evaluated by Ki-67 staining index (SI) was investigated. Results: Five tumors were HI type and seven were LI type. Both progression-free survival after surgical reresection and PDT with TS and overall survival were significantly longer in patients with the LI-type GB compared to the HI-type. The median Ki-67 SI in the area with a signal intensity >5000 arbitrary units (a.u.) was 23.5%, 3000–5000 a.u. was 10.0%, and 1000–3000 a.u. was 2.70%. When the TS signal intensity was 1000 a.u., should be considered for maximum resection of GBs.

Ask AI

Helpful

Bookmark

View Full Paper