Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam

This Data in Brief article presents the draft genome of Pasteurella multocida strain EH32, isolated from a buffalo suspected of having septicemic pasteurellosis in Dak Lak Province, Vietnam. Short-read whole-genome sequencing (2 × 150 bp) was performed on the PacBio Onso platform, using an Illumina-compatible library. The final assembly comprised 2,286,381 base pairs with a GC content of 40.2% across 25 contigs. Genome annotation predicted 2,158 genes, including 2,104 protein-coding sequences, four rRNAs, 49 tRNAs, and one tmRNA. Functional analysis assigned 2,060 genes to COG categories and 1,432 genes to KEGG pathways. Virulence-associated genes involved in adhesion, antiphagocytosis, endotoxin biosynthesis, immune evasion, iron uptake, secretion, and stress response were detected, while no antimicrobial resistance genes were found. A total of 105 carbohydrate-active enzymes were predicted, predominantly glycoside hydrolases and glycosyltransferases. Raw reads and assembly files have been deposited in Mendeley Data and GenBank. This dataset provides a foundational genomic resource to support comparative genomics and pathogenicity research on P. multocida in livestock.