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March 21, 2026Plant Biotechnology Journal0 citationsOpen Access

Engineering Gibberellin-Switchable Dual-Use Line for Hybrid Seed Production in Maize

Engineering a Gibberellin‐Switchable Dual‐Use Line Based on Ent‐Kaurene Oxidase Gene ZmKO1 Enables Two‐Line Hybrid Seed Production in Maize

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Authors

CLChang LiSWSiyu WangYXYong Xiang

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Overview

Experimental study demonstrates novel male sterility traits in maize, indicating effective hybrid seed production strategies.

Key Points

  • The research aims to develop a dual-use line in maize using the ZmKO1 gene for enhanced hybrid seed production.
  • Identified a novel recessive male sterility mutant, def1, linked to the ZmKO1 gene.
  • Conducted map-based cloning and functional analyses of ZmKO1 and ZmKO2 genes.
  • Utilized CRISPR/Cas9 editing to create ZmKO1-based male sterility lines.
  • Applied marker-assisted backcrossing across various genetic backgrounds.
  • The def1 mutant showed non-elongated filaments and normal pollen production.
  • ZmKO1 knockout lines had reduced initial plant height but recovered to wild-type levels.
  • ZmKO2 knockout lines exhibited severe dwarfism and male sterility.
  • The dual-use trait did not significantly affect other agronomic traits.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69be35946e48c4981c673fcahttps://doi.org/10.1111/pbi.70608
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