Synthesis of siRNAs containing carbocyclic nucleotides and the role of cyclopentane conformation in RNAi activity

Jayanta Kundu; Dhrubajyoti Datta; Masaaki Akabane-Nakata; Soham Mandal; Monika Krampert; Martin Egli; Muthiah Manoharan

doi:10.1039/d6cb00038j

January 1, 2026RSC Chemical BiologyOpen Access

Synthesis of siRNAs containing carbocyclic nucleotides and the role of cyclopentane conformation in RNAi activity

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Key Points

The aim is to investigate the synthesis of siRNAs containing carbocyclic nucleotides and their effects on RNA interference (RNAi).
Incorporation of 5′-(E)- and 5′-(Z)-vinylphosphonate carbocyclic DNA into siRNAs
Incorporation of 5′-(E)-vinylphosphonate 2′- and 3′-O-methyl carbocyclic RNAs
Modification of 5′ termini of antisense strands
Successful synthesis of siRNAs with carbocyclic nucleotide modifications
Demonstrated varying effects on RNAi activity based on structural conformation
Highlighted the importance of cyclopentane conformation in enhancing RNAi effects

Implication

Synthesizes siRNAs with carbocyclic nucleotides to assess RNAi activity, highlighting structural significance.

Abstract

5′-( E )- and 5′-( Z )-vinylphosphonate carbocyclic DNA and 5′-( E )-vinylphosphonate 2′- and 3′- O -methyl carbocyclic RNAs were incorporated at 5′ termini of antisense strands of small interfering RNAs.

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Cite This Study

Kundu et al. (2026) studied this question.

synapsesocial.com/papers/69be35f96e48c4981c6747da https://doi.org/10.1039/d6cb00038j

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Synthesis of siRNAs containing carbocyclic nucleotides and the role of cyclopentane conformation in RNAi activity

Key Points

Implication

Abstract

Discussion

Cite This Study

Synthesis of siRNAs containing carbocyclic nucleotides and the role of cyclopentane conformation in RNAi activity

Key Points

Implication

Abstract

Discussion

Cite This Study

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