Comparative analysis of fish embryo acute toxicity (FET) test, fish cell line acute toxicity test and acute fish toxicity test: Evaluating the applicability domain of the FET test
Evaluates the applicability of the fish embryo test in predicting chemical toxicity, suggesting improved methods for assessment.
Key Points
This research aims to explore better methods for predicting the mode of action of chemicals to evaluate the applicability domain of the fish embryo acute toxicity test.
Conducted toxicity tests on 40 chemicals using the fish embryo acute toxicity (FET) test and RTgill-W1 cell line assay.
Included pesticides, pharmaceuticals, and industrial chemicals in the evaluation.
Compared results with the acute fish toxicity (AFT) tests to assess the FET test's applicability domain.
The integrated approach improved predictions of the applicability domain compared to single-scheme methods.
Results showed that the 24-hr median lethal concentration (LC50) from FET reflects basal cytotoxicity.
The ratio of 24-hr LC50 to 96-hr LC50 in FET is useful for estimating modes of action of chemicals.