Comparison of two laser wavelengths for LIBS bioimaging of plants grown in lunar regolith

The colonization of extraterrestrial planets requires sustainable food production independent of Earth-based supplies. Due to the high costs and complicated logistics of food transport, in-situ cultivation will be essential. Growing plants directly in regolith offers a practical approach to achieve sustainable long-term human habitation beyond Earth. In this study, Laser-Induced Breakdown Spectroscopy (LIBS) technique was employed for bioimaging of broccoli ( Brassica oleracea ) and salad ( Lactuca sativa ) plants grown in Lunar regolith simulant and control substrate. For this purpose, the potential of the 2090 nm laser wavelength for bioimaging of plant tissue was studied compared to the conventional 1064 nm. The signal-to-noise ratio (SNR), total emissivity ( ɛ tot ), and Mg II / Mg I intensity ratio (ionization degree) were all higher when using the 2090 nm laser wavelength compared to 1064 nm. These findings indicate that the 2090 nm laser produces a hotter and more efficiently ionized plasma, supporting its feasibility for bioimaging of plant tissues. Additionally, bioimaging with both laser wavelengths has confirmed higher uptake of key plant nutrients such as magnesium (Mg) and calcium (Ca) from Lunar regolith simulant. This results support the potential of LIBS as a diagnostic tool for plant growth monitoring in extraterrestrial environments. • First-time LIBS bioimaging of plants grown in lunar regolith simulants. • Feasibility of using 2090 nm laser for plant bioimaging. • 2090 nm provides higher SNR, total emissivity and ionization efficiency in plant tissue analysis. • Broccoli and salad plants showed elevated Mg and Ca uptake when grown in lunar regolith simulant.