Melatonin as an Epigenetic Modulator to Regulate Angiogenesis in Dalton’s Lymphoma

DLA promote a significant increase in EC proliferation and a high amount of MMP-9 in the presence of DLA. The gelatin zymography also showed the high activity of MMP-2 and MMP-9 with respect to normal ECs. The ROS levels are also found to be high in DLA-induced ECs. The melatonin treatment successfully decreases EC proliferation, MMP-9 levels, and the MMP-2 and MMP-9 activities. The ROS levels were also reduced. The RT-PCR analysis showed that the melatonin modulates the alterations in HDAC1, 2, 6, and 9 and DNMT1, DNMT3A and DNMT3B with respect to the control. The MS-PCR results signify one of the possible ways of action of melatonin on DL cells.