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March 21, 2026New Phytologist2 citationsOpen Access

Soil phosphorus drives subcontinental patterns of carbon isotope discrimination across Australia

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IAIftakharul AlamACAlexander W. CheesmanGFGraham D. Farquhar

Key Points

  • The aim is to explore how soil phosphorus affects carbon isotope discrimination sensitivity in relation to precipitation across Australia.
  • Analysis of existing and new data from transects across Australia
  • Common garden experiments comparing species from phosphorus-rich and phosphorus-poor regions
  • Correlational analysis of Δ 13 C with leaf phosphorus and nitrogen
  • Use of an Australia-wide leaf gas exchange dataset to evaluate intercellular CO2 ratios
  • Δ 13 C sensitivity to mean annual precipitation varies between phosphorus-rich and phosphorus-poor regions.
  • In phosphorus-poor areas, Δ 13 C decreased less with lower mean annual precipitation.
  • Higher stomatal conductance in phosphorus-poor regions moderated decreases in Δ 13 C.
  • Phosphorus availability influenced gas exchange ratios of CO2, affecting carbon isotope discrimination.

Abstract

Summary Several transects have been established to study the sensitivity of carbon isotope discrimination (Δ 13 C) in woody plants to mean annual precipitation (MAP) across Australia. These have shown a surprising divergence in Δ 13 C‐MAP sensitivity among subcontinental regions. We analysed previously reported data alongside new measurements from a transect in northeastern Queensland to explore potential drivers of regional‐scale Δ 13 C‐MAP sensitivity. Multiple lines of evidence indicated this sensitivity is related to soil phosphorus. In phosphorus‐poor regions, Δ 13 C decreased less with decreasing MAP than in phosphorus‐rich regions. Along two contrasting transects in northern Australia, Δ 13 C correlated with leaf phosphorus in the phosphorus‐poor Northern Territory, but not in phosphorus‐rich northeastern Queensland, where it instead correlated with leaf nitrogen. Common garden experiments for species from phosphorus‐poor vs phosphorus‐rich regions showed contrasting relationships between Δ 13 C and species range MAP. Finally, using an Australia‐wide leaf gas exchange dataset, we showed that soil phosphorus influenced the ratio of intercellular to ambient CO 2 concentrations ( c i : c a ), which in turn controls Δ 13 C; the influence was through stomatal conductance, not photosynthetic capacity. Higher stomatal conductance in phosphorus‐poor regions appeared to moderate the decrease in Δ 13 C with decreasing precipitation. We suggest that high transpiration rates in these regions help to facilitate phosphorus foraging in phosphorus‐impoverished, ancient soils.

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Cite This Study

Alam et al. (2026) studied this question.

synapsesocial.com/papers/69be38da6e48c4981c6798cchttps://doi.org/10.1111/nph.71069
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