What question did this study set out to answer?

The research aimed to create and characterize a hiPSC line that can express the rEstus voltage sensor for studying membrane potential dynamics.

March 24, 2026Stem Cell ResearchOpen Access

Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction

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Authors

DMDaniela MalanUniversity of Bonn PRPhilipp RühlJena University Hospital SHStefan H. HeinemannElectrophysiology

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Implication

Characterizes a hiPSC line expressing a voltage sensor, enabling non-invasive membrane potential monitoring in human cells, suggesting potential for drug screening and developmental...

Key Points

The research aimed to create and characterize a hiPSC line that can express the rEstus voltage sensor for studying membrane potential dynamics.
Generated the UKBi015-B-6 hiPSC line via targeted insertion at the AAVS1 locus.
Developed a doxycycline-inducible expression system for rEstus.
Conducted optical electrophysiology techniques to measure membrane potential.
Successfully induced rEstus expression in hiPSCs using doxycycline.
Enabled non-invasive monitoring of membrane potential dynamics.
Facilitated high-throughput studies across various cell types derived from hiPSCs.

Cite This Study

Malan et al. (2026) studied this question.

synapsesocial.com/papers/69c2294caeb5a845df0d38a8 https://doi.org/10.1016/j.scr.2026.103972

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Implication

Characterizes a hiPSC line expressing a voltage sensor, enabling non-invasive membrane potential monitoring in human cells, suggesting potential for drug screening and developmental...

Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction

Discussion

Member takes

Implication

Key Points

Cite This Study

Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction

Discussion

Member takes

Implication

Key Points

Cite This Study

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