A Platform to Design and Optimise Fluorogenic scFvs for Detection of Interleukin 33

Key Points

• The aim is to create a platform for discovering and optimizing fluorogenic single-chain variable fragments (scFvs) to measure interleukin-33.
• Developed a novel platform for fluorescence-based detection of IL-33
• Optimized scFvs for enhanced sensitivity and specificity
• Conducted comparative analyses with existing detection methods
• Successfully developed fluorogenic scFvs that effectively detect IL-33
• Achieved higher sensitivity than traditional detection methods
• Provided a useful tool for studying IL-33 in inflammatory diseases