This research demonstrates changes in microbial communities in anaerobic digesters due to Caldicellulosiruptor bescii pretreatment of wastewater substrates, indicating potential improvements in...
We report 16S rDNA sequencing of anaerobic digester microbiomes processing primary clarifier sludge, anaerobic digestate, and waste activated sewage with and without biological pretreatment of Caldicellulosiruptor bescii. DNA was extracted from pellet-associated and free-living microhabitats. Amplicon sequencing with Illumina MiSeq was performed targeting the V4 region of 16S rDNA.
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Nipko et al. (2026) studied this question.
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