The current study determined that artificial seawater generation techniques are adequate for maintaining economically valuable seaweeds in a laboratory environment. It was discovered that in the artificial seawater formulation, Gracilaria salicornia grew more quickly than the control. The physical and chemical characteristics of the synthetic saltwater, including its salinity, pH, phosphate, nitrate, photosynthesis, respiration, and chlorophyll levels, were contrasted with those of the control (crude seawater). The seaweed's anticancer properties were assessed by comparing the morphology and cell viability of the treated and control cells. With an IC of 231.00 μg/ml.69 against the A375 cell line, G. salicornia exhibited anticancer activity. Comparing the treated cells' morphology to that of the control cells revealed significant differences. According to the in vitro assay, the development of Gracilaria salicornia in a red seaweed artificial saltwater medium is a substantial source of a noble anticancer agent. Additionally, G. salicornia may soon be one of the most promising bioagents for cancer treatment, according to the study.
J et al. (Sat,) studied this question.