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March 26, 2026BMC BiotechnologyOpen Access

Systematic engineering of Escherichia coli for biosynthesis of 3-hydroxypropionic acid from glucose and malonate

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Authors

YWYingying WangMCMeihui CaoMHMei Hu

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Overview

Demonstrates enhanced 3-HP biosynthesis in engineered Escherichia coli, suggesting a scalable microbial platform for industrial applications.

Key Points

  • The aim is to develop a genetically engineered strain of Escherichia coli for improved biosynthesis of 3-hydroxypropionic acid (3-HP).
  • Engineered Escherichia coli by integrating malonate assimilation and 3-HP biosynthesis genes using CRISPR/Cas9.
  • Repressed fatty acid biosynthesis genes via CRISPR interference to improve 3-HP yield.
  • Introduced a malonyl-CoA-responsive biosensor for dynamic regulation of gene expression.
  • Conducted fermentation in a fed-batch bioreactor to optimize conditions.
  • Achieved maximum 3-HP production of 42.22 g/L with a specific productivity of 0.69 g/g.
  • Produced 21.97 mM 3-HP, representing a 0.51-fold increase over plasmid-based systems.
  • Increased 3-HP titer by 66% through fatty acid biosynthesis gene repression.
  • Enhanced production by 59% with the implementation of the FapR/fapO biosensor.

Cite This Study

Wang et al. (2026) studied this question.

synapsesocial.com/papers/69c4cda5fdc3bde44891a4c6https://doi.org/10.1186/s12896-026-01140-2
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