Mutagenesis-Based Evolution Enables Rapid Retargeting of L-Aptamers for Structured RNA Recognition

Xuan Han; Tyler Guilbault; Jonathan T. Sczepanski

doi:10.1039/d6cb00057f

January 1, 2025RSC Chemical BiologyOpen Access

Mutagenesis-Based Evolution Enables Rapid Retargeting of L-Aptamers for Structured RNA Recognition

View Full Paper

Ask AI

Bookmark

Share

Key Points

This research aims to enhance the specificity of L-aptamers for recognizing structured RNAs using mutagenesis-based evolution.
Utilized mirror-image L-aptamers to target structured RNAs
Employed mutagenesis to create diverse aptamer libraries
Conducted selection processes based on binding affinity and specificity
Demonstrated improved recognition of structured RNAs by retargeted L-aptamers
Achieved higher binding affinities compared to traditional methods
Highlighted the effectiveness of cross-chiral interactions in targeting RNA structures

Implication

Mutagenesis enables high-affinity RNA recognition in structured RNA, suggesting new therapeutic approaches.

Abstract

Mirror-image L-(deoxy)ribose nucleic acid aptamers (L-aptamers) enable high-affinity, selective recognition of native structured RNAs via shape-based, cross-chiral interactions rather than Watson–Crick base pairing. However, achieving this level of specificity has...

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Han et al. (2025) studied this question.

synapsesocial.com/papers/69c6207d15a0a509bde18ee1 https://doi.org/10.1039/d6cb00057f

View Full Paper

Ask AI

Bookmark

Share

Mutagenesis-Based Evolution Enables Rapid Retargeting of L-Aptamers for Structured RNA Recognition

Key Points

Implication

Abstract

Discussion

Cite This Study

Mutagenesis-Based Evolution Enables Rapid Retargeting of L-Aptamers for Structured RNA Recognition

Key Points

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading