Experiment reveals effects of solvent polarity on fluorescence emission, suggesting key principles of spectroscopy.
This article describes an experiment that can be conducted to cover basic fluorescence concepts and study the effects of solvent polarity. It introduces students to fluorescence spectrophotometry and illustrates the effects of the solvent’s dielectric constant and hydrogen bonding capability on fluorescence-emission spectra.
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Barghouthi et al. (1998) studied this question.
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