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Undergraduate exercise demonstrates mixture analysis using UV spectroscopy and HPLC, indicating effective educational methods.
An exercise is described for the analysis of a mixture containing acetaminophen, aspirin, and caffeine using UV spectroscopy and HPLC. The concentrations of various components in a mixture are determined directly from the UV spectra using multiple linear regression, and then the concentrations of various components in a mixture are determined using HPLC data. The experiment is appropriate for undergraduate students in analytical chemistry.
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Smith et al. (2010) studied this question.
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