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March 29, 2026Journal of Biological EngineeringOpen Access

High-titer 2-phenylethanol production in Escherichia coli via decarboxylation-step optimization and a vegetable-oil overlay for biocompatible in situ recovery

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Authors

CLChangli LiuHZHongwei ZhangWXWanbin Xing

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Overview

Identifies a new pathway to enhance 2-phenylethanol production in E. coli, suggesting improved bioproduction methods.

Key Points

  • The research aims to optimize the production of 2-phenylethanol in E. coli by addressing the decarboxylation bottleneck.
  • Optimized the Ehrlich pathway to convert l-phenylalanine to 2-phenylethanol.
  • Used whole-cell assays to identify bottlenecks in the decarboxylation process.
  • Substituted yeast decarboxylase Aro10 with Lactococcus lactis KivD.
  • Incorporated a soybean oil overlay for in situ product recovery.
  • Employed a fed-batch feeding strategy to enhance product yield.
  • Achieved 49.5 mM 2-phenylethanol from 50 mM l-phenylalanine with 99.0% conversion.
  • Produced 130 mM of 2-phenylethanol (15.9 g/L) at shake-flask scale.
  • Increased overall recovery yield to 94.2% based on l-phenylalanine consumption using soybean oil.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/69c8c384de0f0f753b39e59ahttps://doi.org/10.1186/s13036-026-00667-4
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