High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

Q: What does this research mean for the field?

A colorimetric LAMP assay using cresol red, combined with an intelligent smartphone application, enables automated, high-throughput visual detection of Mycoplasma gallisepticum with 98% concordance to standard qPCR. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Wuyang Jing; Qianyi Cai; Yucen Liang; Renhang Xiao; Qijie Lin; X. George Xu; Ruidong Liu; Chenggang Xu; Hongchao Gou; Haiyan Shen; Ming Liao; Jianmin Zhang

doi:10.1039/d6ay00198j

January 1, 2026Analytical Methods

High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

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Key Points

To develop a rapid and reliable method for detecting Mycoplasma gallisepticum using colorimetric LAMP and smart technology.
Utilized a colorimetric LAMP assay with cresol red for visual detection.
Developed a smartphone application (Lab Color Analyzer) for automated sample analysis.
Tested the assay performance against qPCR for accuracy.
Achieved 98% concordance with qPCR for detecting Mycoplasma gallisepticum.
Enabled analysis of up to 64 samples simultaneously.
Provided rapid visual detection of the bacteria.

Implication

Demonstrates a colorimetric LAMP assay for Mycoplasma gallisepticum, indicating reliable and fast detection through smart technology.

Abstract

A colorimetric LAMP assay using cresol red enables visual detection of Mycoplasma gallisepticum , while an intelligent smartphone application (Lab Color Analyzer) automates analysis of up to 64 samples, achieving 98% concordance with qPCR.

Discussion

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Cite This Study

Jing et al. (2026) studied this question.

synapsesocial.com/papers/69c8c3a8de0f0f753b39ea18 https://doi.org/10.1039/d6ay00198j

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High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

Key Points

Implication

Abstract

Discussion

Cite This Study

High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

Key Points

Implication

Abstract

Discussion

Cite This Study

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