What type of study is this?

This is a In Vitro Study study.

What question did this study set out to answer?

To evaluate the in vitro antioxidant activity of Enicostemma littorale leaf extracts.

March 30, 20260 citationsOpen Access

Phytochemical Profiling and Comparative Antioxidant Potential of Enicostemma Littorale Leaf Extracts Using in Vitro Assays

CPChennu M. M. Prasada Rao1*, Tanniru Rajeswari2, Jyotirmayee Patel2, Renuka Barik2, Rupashree Nishad2, Prakash Pradhan2

Key Points

To evaluate the in vitro antioxidant activity of Enicostemma littorale leaf extracts.
Assessed antioxidant activity using DPPH radical scavenging assay and reducing power assay.
Conducted preliminary phytochemical screening for alkaloids, flavonoids, phenolics, and tannins.
Calculated total phenolic content (TPC) and IC₅₀ values for both extracts.
Methanolic extract showed 30.2% inhibition at 100 µg/mL and 74.2% at 800 µg/mL in the DPPH assay.
Aqueous extract exhibited 22.4% inhibition at 100 µg/mL and 62.5% at 800 µg/mL.
Methanolic extract had an IC₅₀ of approximately 280 µg/mL, while aqueous extract had an IC₅₀ of 500 µg/mL.

Abstract

The present study was carried out to evaluate the in vitro antioxidant activity of methanolic and aqueous leaf extracts of Enicostemma littorale. Antioxidant potential was assessed using DPPH radical scavenging assay, reducing power assay, and total phenolic content (TPC) estimation. Preliminary phytochemical screening confirmed the presence of alkaloids, flavonoids, phenolics, and tannins in both extracts.The DPPH assay showed a concentration-dependent increase in radical scavenging activity. The methanolic extract exhibited 30.2% inhibition at 100 µg/mL and 74.2% at 800 µg/mL, while the aqueous extract showed 22.4% and 62.5%, respectively. The IC₅₀ values were calculated using linear interpolation as approximately 280 µg/mL for the methanolic extract and 500 µg/mL for the aqueous extract, indicating higher antioxidant potency of the methanolic extract. The total phenolic content was calculated using the calibration equation , yielding values of 88 mg GAE/g extract for the methanolic extract and 64 mg GAE/g extract for the aqueous extract. The reducing power assay also demonstrated increased absorbance with concentration, confirming strong electron-donating ability.

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