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Quantifies quercetin in Blumea balsamifera leaves, suggesting significant bioactive properties.
Quercetin, a flavonoid compound widely distributed in plants, is considered beneficial physiologically due to attributed bioactivity such as anti-cancer, immunomodulatory, antidiabetic, and anti-inflammatory. In this study, the quercetin content from the dried Blumea balsamifera L. DC dried leaf was macerated with 95% ethanol and the concentrated extract was purified using the Modified Kupchan method and flash chromatography. All fractions were tested for the presence of flavonoids using phytochemical screening and the selected dichloromethane fraction was further purified using another round of flash chromatography. All resulting fractions and pooled samples were tested for the antioxidant property using the developed Thin Layer Chromatography (TLC)-Bioautography and separated compounds were derivatized with DPPH. Using the optimized TLC-Bioautography method, the quercetin content in the dichloromethane fraction was analyzed and compared with a reversed-phase high-performance liquid chromatography hyphenated with photodiode array detector (RP-HPLC-PDA). The calculated quercetin content from the pooled sample using TLC-bioautography method was 2.25 mg/ml and from RP-HPLC-PDA was 2.02 mg/ml which was statistically uncomparable using unpaired t-test (p<0.05, α=0.05).
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Kamantigue et al. (2022) studied this question.
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