Micelle-Assisted Cloud Point Extraction and Spectrophotometric Determination of Silver Ions Using a Newly Synthesized 2-[(benzimidazole)azo]-4-methylphenol Reagent

Herein, the new azo reagent, 2-[(benzimidazole)azo]-4-methylphenol (BIAP), was created and used as a chromophore reagent. BIAP's structure was identified using UV-Vis, FTIR and 1 H NMR. The BIAP was used to extract silver ions from water and foods. A simple cloud point extraction (CPE) approach for the determination of silver ions by a spectrophotometer detector was applied. It is worth noting that the use of TritonX-100 (TX-100) improved the method's sensitivity and stability following chelation with silver ion. In addition, the chelation of silver ion and the azo reagent was measured spectroscopically using two methods (UV-Vis and FAAS). The spectrophotometric method determines the ion-pair association complex of silver at λ max = 594 nm. Additionally, the optimum critical volume of TX-100, temperature, and heating time were 0.6 mL, 85°C, and 15.0 min, respectively. The thermodynamic functions are calculated as follows: ΔH ex = 0.0783 KJ mol -1 , ΔG ex = –67.3978 KJ mol -1 , and ΔS ex = 188.4807 J K -1 mol -1 . The LOD, PF, EF, and linearity bands were 0.26 μg L -1 , 166.66, 22.46, and 1.0 -15.0 μg L -1 , respectively.