Development and Validation of Uv-Visible Spectroscopic Method for the Estimation of Bisoprolol in Bulk and Pharmaceutical Dosage Form

UV Spectroscopy is a widely used analytical technique that involves the absorption of ultraviolet radiation by molecules, leading to electronic transitions. The aim of the presented work is to develop and validate a simple, rapid, accurate, precise and effective UV-spectroscopy method for the estimation of bisoprolol, an anti- hypertensive drug. Ethanol is used as the solvent for the preparation of stock solution as it shows good solubility when compared with the other solvents. The wavelength at maximum absorption (λ max) was found to be 273nm. The drug bisoprolol shows the linearity concentration range 20-180µg with the correlation coefficient value of 0. 9961and a regression equation y =0. 004x+ 0. 0311. Then a graph was plotted by taking the concentration x- axis and absorbance on y-axis which gives straight line percentage assay of bisoprolol in pharmaceutical formulation was found to be 99. 58%. The LOD and LOQ was found to be 0. 284 & 0. 863 respectively. Precision studies show acceptable results with intraday precision and interday precision as %RSD <2 method validation was carried out in accordance with international council of harmonization guidelines.