Production of Metastases by Treatment with Carcinostatic Agents : III. Low Concentration of Carcinostatic Agents on the Cells

The effects of treatment with various doses of carcinostatic agents on tumor growth have been studied. In animal experiments. treatment with low concentrations of nitrogen mustard N-oxide resulted in an early death of mice bearing Ehrlich ascites carcinoma and an increase in the number of circulating Yoshida sarcoma cells. The growth of subcutaneously implanted Yoshida sarcoma was stimulated after contact of the cells with low concentrations of nitrogen mustard N-oxide in vitro or intraperitoneally. Low concentrations of Mitomycin-C and Chromomycin A3 also showed increase of tumor growth and metastasis formation. In vitro study showed that the proliferation of HeLa cells was stimulated by adding low concentrations of nitrogen mustard N-oxide. Mitomycin-C, and Chromomycin A3 into the culture media. In clinical studies, much more cancer cells were released into the stomach cavity after the administration of nitrogen mustard N-oxide, Mitomycin-C, and Chromomycin A3.