New developingand Validation method to Determine Lidocaine Hydrochloride in Raw Material and Injection Forms by Using RP-HPLC

This work was aimed to develop a rapid, simple, sensitive and precise high performance liquid chromatography (HPLC) method for the estimation of Lidocaine hydrochloride (Lido) in both injection dosage forms and raw materials. The chromatographic separation was achieved with acetonitrile:phosphoric acid (pH = 3) in ratio of 30:70 (v/v) as mobile phase on a Shim-pack clc-C8 column (25 cm x 4.6 mm i.d., 5 μm) with UV detection at 212 nm, pump flow rate 1.0 mL/min and sample injection volume 20 μL. The method was validated with respect to linearity, precision, accuracy and specificity. The calibration curve showed good linearity over the concentration range of (2.5 - 250) µg/mL in presence of Caffeine (Caff) as internal standard with limit of detection and limit of quantification were to be 0.31 𝜇g/mL and 0.94 𝜇g/mL, respectively. The developed method was very rapid with a run time of 4.5 min and found to be successively applied for the quality control of (Lido) in pharmaceutical formulations