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April 3, 2026Nature CommunicationsOpen Access

Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling

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Authors

HDHelen DaubenMMMihaela MihaljevićAKAndreas Kolvenbach

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Overview

Demonstrates enhanced PARP1 signalling in XRCC1-deficient cells, indicating key modifications during DNA damage.

Key Points

  • The aim is to investigate the effects of XRCC1 deficiency on ADP-ribosylation in the context of DNA damage.
  • Developed antibodies for specific detection of mono- and poly(ADP-ribosyl)ation
  • Utilized mass spectrometry to map poly(ADP-ribosyl)ation sites
  • Conducted experiments on DNA damage response in XRCC1-deficient cells
  • XRCC1 deficiency increases mono-ADP-ribosylation during DNA damage response
  • PARP1 hyperactivation is observed in XRCC1-deficient contexts
  • Enhanced recruitment of RNF114 to DNA lesions was detected due to modified ubiquitylation

Cite This Study

Dauben et al. (2026) studied this question.

synapsesocial.com/papers/69cf5ebd5a333a821460d4c3https://doi.org/10.1038/s41467-026-71311-4
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