Abstract 3153: An integrated in vitro profiling platform for the simultaneous assessment of anti cancer efficacy and multi organ toxicity

Key Points

• The aim is to create a platform that simultaneously assesses anti-cancer efficacy and organ-specific toxicity during drug development.
• Developed an integrated in vitro profiling platform.
• Utilized a High-Throughput Viability Screening across human cancer cell lines.
• Implemented functional phenotyping assays for anti-metastatic evaluation.
• Conducted molecular investigations using qPCR and Western Blot analysis for mechanism elucidation.
• Employed specialized assays in normal cell models to identify organ-specific toxicities.
• Identified the potency and selectivity of anti-tumor compounds through the efficacy module.
• Evaluated anti-metastatic potential via angiogenesis, migration, and invasion assays.
• Proactively identified various organ-specific toxicities including hepatotoxicity and nephrotoxicity.
• Integrated findings to support safer candidate selection for drug development.