What question did this study set out to answer?

The study aims to develop and validate bioPROTACs that target and degrade essential viral proteins in porcine arterivirus.

April 7, 2026Journal of Nanobiotechnology0 citationsOpen Access

Nanobody-based bioPROTAC for viral protein degradation provides an antiviral strategy for porcine arterivirus

SSShibo SuChinese Academy of Agricultural Sciences MSMingxia SunChinese Academy of Agricultural Sciences HWHaiwei WangSouthwest University

Key Points

The study aims to develop and validate bioPROTACs that target and degrade essential viral proteins in porcine arterivirus.
Constructed bioPROTACs by fusing nanobodies to the BTB domain of SPOP.
Utilized mRNA-LNPs for effective delivery of bioPROTACs.
Tested the impact of bioPROTACs on viral replication in a pig model.
BioPROTACs targeting various stages of viral replication significantly suppressed viral replication.
Demonstrated potential for use against multiple lineages of porcine arterivirus.
Showed mechanisms of target degradation through the SPOP pathway.

Abstract

The aim of the current study was to develop and validate modular bioPROTACs targeting essential viral proteins. We constructed the degraders by fusing target-specific nanobodies to the BTB domain of SPOP. More importantly, a combination of bioPROTACs targeting different stages of viral replication, delivered via mRNA-LNPs, suppressed viral replication in a pig model. These findings offer valuable insights into the target degradation mechanisms of SPOP-derived bioPROTACs and provide a foundation for the design of antivirals that have activity against multi-lineages of porcine arterivirus and overcome drug resistance.

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