June 22, 2012Circulation Research195 citationsOpen Access
View Full PaperStimulated Emission Depletion Live-Cell Super-Resolution Imaging Shows Proliferative Remodeling of T-Tubule Membrane Structures After Myocardial Infarction
Structured PICO
P
Population
Preclinical model of early heart failure (HF) development after myocardial infarction (specific animal/cell model not stated in abstract)
I
Intervention
Stimulated Emission Depletion (STED) nanoscopy for live mapping of T-tubule (TT) membrane structures
O
Outcome
T-tubule (TT) morphology, associated proteins, network remodeling, and Ca(2+) release dyssynchronysurrogate
Live-cell STED nanoscopy demonstrates that early heart failure development after myocardial infarction is characterized by proliferative T-tubule membrane changes, network fracturing, and excitation-contraction uncoupling.
Abstract
This study introduces STED nanoscopy for live mapping of TT membrane structures. During early HF development, the local TT morphology and associated proteins were significantly altered, leading to differential network remodeling and Ca(2+) release dyssynchrony. Our data suggest that TT remodeling during HF development involves proliferative membrane changes, early excitation-contraction uncoupling, and network fracturing.