Why the study?
Does a fluorogenic probe hydrolysis (TaqMan) PCR assay detect African swine fever virus in experimentally infected swine prior to clinical disease?
Does a fluorogenic probe hydrolysis (TaqMan) PCR assay detect African swine fever virus in experimentally infected swine prior to clinical disease?
A real-time PCR assay can detect African swine fever virus in swine 2 to 4 days before clinical symptoms appear, aiding in preclinical diagnosis and outbreak management.
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A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.
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May aid preclinical ASFV surveillance in swine; leaves open validation in natural infections and field settings.
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Zsak et al. (2005) studied this question.
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